A wide dynamic range detection of biopolymer medicines with resonance light scattering and absorption ratiometry [An article from: Analytica Chimica Acta]

by Y.J. Long, Y.F. Li, C.Z. Huang

List Price:	~~$8.95~~
Amazon Price:	$8.95 & eligible for FREE Super Saver Shipping on orders over $25.
Lowest New Price:	$8.95
Availablitiy:	Available for download now

Editorial Reviews
Product Description
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in . The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

Description:
Using the interaction of heparin with Janus Green Blue (JGB) as an example, herein a dual-wavelength resonance lighting scattering (RLS) ratiometric method and an absorbance ratiometric one are developed to detect biopolymer medicines based on their bindings with organic dyes. In aqueous solution, heparin could interact with JGB, displaying significantly enhanced RLS signals in UV-vis region. By measuring the RLS intensity ratio (R"r"a"t"i"o) and absorbance ratio (A"r"a"t"i"o) at the wavelengths of 285nm over that at 345nm, respectively, heparin over a wide dynamic range of content could be detected. Typically, when JGB concentration is kept at 1.00x10^-^5moll^-^1, using R"r"a"t"i"o could detect heparin over a range of 0.30-2000ngml^-^1 with a limit of determination of 30pgml^-^1, while using A"r"a"t"i"o could detect heparin over the range of 0.01-2000.0ngml^-^1 with a limit of determination of 1pgml^-^1. The wavelength-dependent RLS and absorption ratiometric methods were found to have much better flexibility for a series of common influences, such as pH, and dye concentration, than that of RLS method at a single wavelength. The interaction nature was investigated through size measurements and RLS imaging detections.

Price is accurate as of the date/time indicated. Prices and product availability are subject to change. Any price displayed on the Amazon website at the time of purchase will govern the sale of this product.